Background: Akabane disease (AD), a barrier to international trade for endemic areas with far economic impact\non the countries, is caused by Akabane virus (AKAV). Commercial enzyme-linked immunosorbent assay (ELISA) is a\ncommonly used diagnostic technique for AKAV infection, including the IDEXX and IDVET ELISA kits. However, the\ncomparative evaluation of the IDEXX and IDVET ELISA kits has not been published. The object of this study was to\nevaluate the test performance of the two commercial ELISA kits in detecting serum anti-AKAV antibodies in cattle.\nResults: With virus neutralization test (VNT) as the â??relative gold standardâ?, the diagnostic sensitivity (DSe) was\n80.39% (123/153) and 93.46% (143/153) for the IDEXX and IDVET ELISA kit, when suspect samples were included.\nThe diagnostic specificity (DSp) for the IDEXX and IDVET ELISA kit was 93.48% (502/537) and 82.31% (442/537),\nrespectively.\nConclusion: Both of the tested ELISA kits could be applied to detect antibodies against AKAV in cattle serum. The\nIDVET ELISA kit had a higher DSe. The IDEXX ELISA kit possessed the higher DSp. These results have important\nimplications if the kits are used to screen herds or individual cattle in surveillance programs, or at border crossings\nfor import-export inspection and quarantine.
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